Bovine Pestivirus Infection: a Cause of Ovulatory Disturbance in Dairy Cows

نویسندگان

  • H. Bielefeldt-Ohmann
  • M. Kafi
  • M.R. McGown
چکیده مقاله:

  Twelve (Experiment I) and four (Experiment II) multiparous dairy cows seronegative to pestivirus were selected and randomly assigned to either a control group which did not become infected or a treatment group in which all cows became infected following intranasal inoculation 9 days before AI. The experimental induction of infection was carried out with 2 ml of non-cytopathic pestivirus (BVD virus) suspension containing 5 log10 TCID50/ml (Experiment I) and 4.5 log10 TCID50/ml (Experiment II). In both experiments, the cows were superovulated on day 10±2 of the cycle using the standard procedures. The cows in Experiment I were artificially inseminated at 12 and 24 h after the onset of estrus and a non-surgical ova/embryo collection was performed 7 days after AI. In Experiment II, the cows were slaughtered on day 8 after superovulation-induced estrus and the ovaries submitted for gross and histopahological examination including immunohistochemistry. Mean (±SE) number of ovulatory sized follicles on day of AI and corpora lutea palpated on day 7 after AI were significantly (p

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Bovine pestivirus infection: a cause of ovulatory disturbance in dairy cows

  Twelve (Experiment I) and four (Experiment II) multiparous dairy cows seronegative to pestivirus were selected and randomly assigned to either a control group which did not become infected or a treatment group in which all cows became infected following intranasal inoculation 9 days before AI. The experimental induction of infection was carried out with 2 ml of non-cytopathic pestivirus (BVD ...

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bovine pestivirus infection: a cause of ovulatory disturbance in dairy cows

twelve (experiment i) and four (experiment ii) multiparous dairy cows seronegative to pestivirus were selected and randomly assigned to either a control group which did not become infected or a treatment group in which all cows became infected following intranasal inoculation 9 days before ai. the experimental induction of infection was carried out with 2 ml of non-cytopathic pestivirus (bvd vi...

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bovine pestivirus infection: a cause of ovulatory disturbance in dairy cows

twelve (experiment i) and four (experiment ii) multiparous dairy cows seronegative to pestivirus were selected and randomly assigned to either a control group which did not become infected or a treatment group in which all cows became infected following intranasal inoculation 9 days before ai. the experimental induction of infection was carried out with 2 ml of non-cytopathic pestivirus (bvd vi...

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عنوان ژورنال

دوره 61  شماره 1

صفحات  22- 32

تاریخ انتشار 2006-01-01

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